| Title | Denaturing gradient gel electrophoresis and multi-SIR profiles of soil microbial communities from a karst doline at Aggtelek National Park, Hungary |
| Publication Type | Journal Article |
| Year of Publication | 2021 |
| Authors | Mucsi M., Krett G., Szili-Kovacs T., Moga J., Borsodi A.K |
| Journal | Folia Microbiologica |
| Volume | 66 |
| Pagination | 107-114 |
| Date Published | Feb |
| Type of Article | Article |
| ISBN Number | 0015-5632 |
| Accession Number | WOS:000578890200001 |
| Keywords | Bacteria, biodiversity, Biotechnology & Applied Microbiology, DGGE, diversity, Karst soil, Microbiology, MicroResp, MICRORESP(TM), Multi-SIR, respiration, rhizosphere |
| Abstract | Soils play an important role in the ecosystem of karstic landscapes both as a buffer zone and as a source of acidity to belowground water. Although the microbiota of karstic soils is known to have a great effect on karstification processes, the activity and composition of these communities are largely unknown. This study gives a comparative analysis of soil microbial profiles from different parts of a doline located at Aggtelek, Hungary. The aim was to reveal the relationships between the vegetation type and genetic fingerprints and substrate utilisation (multi-SIR) profiles of the soil microbiota. Soil samples were collected in early and late springs along a transect in a doline covered with different types of vegetation. Genetic fingerprints of bacterial communities were examined by denaturing gradient gel electrophoresis (DGGE) based on the 16S rRNA gene, along with multi-SIR profiles of the microbial communities measured by the MicroResp method using 15 different carbon sources. Genetic fingerprinting indicated that vegetation cover had a strong effect on the composition of soil bacterial communities. Procrustean analysis showed only a weak connection between DGGE and multi-SIR profiles, probably due to the high functional redundancy of the communities. Seasonality had a significant effect on substrate usage, which can be an important factor to consider in future studies.
|
| Short Title | Folia Microbiol.Folia Microbiol. |
| Alternate Journal | Folia Microbiol. |
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Mucsi, Marton Krett, Gergely Szili-Kovacs, Tibor Moga, Janos Borsodi, Andrea K.
Szili-Kovacs, Tibor/AAJ-9680-2020; Borsodi, Andrea/H-1148-2017
Szili-Kovacs, Tibor/0000-0002-8354-0656; Mucsi, Marton/0000-0001-6226-0607; Borsodi, Andrea/0000-0002-3738-7937; Moga, Janos/0000-0002-4471-4385
ELKH Centre for Agricultural Research; Hungarian Scientific Research Fund (OTKA)Orszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [T79135]
Open access funding provided by ELKH Centre for Agricultural Research. This research was supported by the Hungarian Scientific Research Fund (OTKA, Grants No. T79135).
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Springer
Dordrecht
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Szili-Kovacs, T (corresponding author), Ctr Agr Res, Inst Soil Sci & Agr Chem, Herman Otto Ut 15, H-1022 Budapest, Hungary.
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