Influence of invasive earthworms (Amynthas spp.) on Wisconsin forest soil microbial communities and soil chemistry

TitleInfluence of invasive earthworms (Amynthas spp.) on Wisconsin forest soil microbial communities and soil chemistry
Publication TypeJournal Article
Year of Publication2020
AuthorsPrice-Christenson G.J, Johnston M.R, Herrick B.M, Yannarell A.C
JournalSoil Biology and Biochemistry
Volume149
Pagination11
Date PublishedOct
Type of ArticleArticle
ISBN Number0038-0717
Accession NumberWOS:000567094800033
Keywordsagrestis goto, Agriculture, Amynthas agrestis, Amynthas tokioensis, asian earthworms, carbon, champlain valley, community, Earthworm, enzyme-activity, Hardwood forests, impacts, Invasion, lumbricus, MICROBIAL, organic-matter, TEMPERATE
Abstract

The invasion of Amynthas species complex of earthworms from Asia into North American hardwood forests poses a novel threat to these ecosystems. These species can competitively exclude previously-established earthworms, have novel effects on microbial communities, and influence the structural and chemical composition of soils differently than other earthworm species. However, because prior research has found that earthworm species is an important factor for microbial community composition, Amynthas species may also uniquely affect soil microbial community composition. To address this, we took earthworm and soil samples from 0.5-m quadrats along an active invasion front of Amynthas agrestis and A. tokioensis at the University of Wisconsin-Madison Arboretum. We tested all soil samples for basal metabolic rates, total carbon, and total nitrogen. We also extracted microbial DNA from cast samples, dissected worm guts, and soil samples, and we used high-throughput DNA sequencing to characterize Amynthas species-specific differences in bacterial and fungal community composition. We found that plots that had been occupied by Amynthas for longer than a year had higher total nitrogen, lower C/N, and different communities of soil bacteria and fungi when compared to newly-invaded plots. We also found that Amynthas had species-specific differences in bacterial communities adhered to worm guts and in worm casts. These results suggest that Amynthas species introduce novel assemblages of bacteria and fungi through their casts, and these differences are associated with the species-specific community composition of earthworm guts. These results also suggest that Amynthas species are likely affecting the soil microbial community during invasion.

Short TitleSoil Biol. Biochem.Soil Biol. Biochem.
Alternate JournalSoil Biol. Biochem.
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Times Cited: 1
Cited Reference Count: 55
Price-Christenson, Gabriel J. Johnston, Marie R. Herrick, Bradley M. Yannarell, Anthony C.
USDA National Institute of Food and Agriculture by the Hatch Act of 1887 project [1004684]; AFRI Foundational and Applied Science Program [2018-67013-27537]; Cooperative State Research, Education, and Extension ServiceUnited States Department of Agriculture (USDA)National Institute of Food and Agriculture [ILLU 875-952]
I would like to thank my wife Suzanne for sacrificing her weekends to help sample earthworms with me and Alex Kent and Lora Cheng for helping to process samples. I would also like to thank Tricia Bethke, Christopher Evans, and Martha Hellander for their advice, feedback, and expertise. This work was supported through the USDA National Institute of Food and Agriculture by the Hatch Act of 1887 project accession no. 1004684, AFRI Foundational and Applied Science Program grant no. 2018-67013-27537, and the Cooperative State Research, Education, and Extension Service under project number ILLU 875-952.
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Price-Christenson, GJ (corresponding author), Edward R Madigan Lab, Room 310 1202 W Gregory Dr, Urbana, IL 61801 USA.
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