Mycorrhizal effector PaMiSSP10b alters polyamine biosynthesis in Eucalyptus root cells and promotes root colonization

TitleMycorrhizal effector PaMiSSP10b alters polyamine biosynthesis in Eucalyptus root cells and promotes root colonization
Publication TypeJournal Article
Year of Publication2020
AuthorsPlett J.M, Plett K.L, Wong-Bajracharya J., Pereira M.D, Costa M.D, Kohler A., Martin F., Anderson I.C
JournalNew Phytologist
Volume228
Pagination712-727
Date PublishedOct
Type of ArticleArticle
ISBN Number0028-646X
Accession NumberWOS:000572124100028
Keywordscarbon-dioxide, ectomycorrhizal, ectomycorrhizal fungus, Fungi, immunity, infection, laccaria-bicolor, mutualistic symbiosis, nutrition, Pisolithus, Plant Sciences, plant-microbe interactions, plants, protein-interaction, resistance, s-adenosylmethionine-decarboxylase, spermidine synthase, symbiosis
Abstract

Pathogenic microbes are known to manipulate the defences of their hosts through the production of secreted effector proteins. More recently, mutualistic mycorrhizal fungi have also been described as using these secreted effectors to promote host colonization. Here we characterize a mycorrhiza-induced small secreted effector protein of 10 kDa produced by the ectomycorrhizal fungusPisolithus albus, PaMiSSP10b. We demonstrate that PaMiSSP10b is secreted from fungal hyphae, enters the cells of its host,Eucalyptus grandis,and interacts with an S-adenosyl methionine decarboxylase (AdoMetDC) in the polyamine pathway. Plant polyamines are regulatory molecules integral to the plant immune system during microbial challenge. Using biochemical and transgenic approaches we show that expression of PaMiSSP10b influences levels of polyamines in the plant roots as it enhances the enzymatic activity of AdoMetDC and increases the biosynthesis of higher polyamines. This ultimately favours the colonization success ofP. albus. These results identify a new mechanism by which mutualistic microbes are able to manipulate the host ' s enzymatic pathways to favour colonization.

Short TitleNew Phytol.New Phytol.
Alternate JournalNew Phytol.
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Times Cited: 2
Cited Reference Count: 76
Plett, Jonathan M. Plett, Krista L. Wong-Bajracharya, Johanna de Freitas Pereira, Maira Costa, Mauricio Dutra Kohler, Annegret Martin, Francis Anderson, Ian C.
; MARTIN, Francis/G-2822-2013
Wong, Johanna Wing-Hang/0000-0001-6119-8645; Anderson, Ian/0000-0002-3507-163X; Plett, Jonathan/0000-0003-0514-8146; MARTIN, Francis/0000-0002-4737-3715; Plett, Krista/0000-0001-6422-3754
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