| Title | A Multi-biological Assay Approach to Assess Microbial Diversity in Arsenic (As) Contaminated Soils |
| Publication Type | Journal Article |
| Year of Publication | 2017 |
| Authors | Stazi S.R, Moscatelli M.C, Papp R., Crognale S., Grego S., Martin M., Marabottini R. |
| Journal | Geomicrobiology Journal |
| Volume | 34 |
| Pagination | 183-192 |
| Type of Article | Article |
| ISBN Number | 0149-0451 |
| Accession Number | WOS:000394650300010 |
| Keywords | Arsenic, bacterial communities, biomass, DGGE, EL-FAME, Environmental Sciences & Ecology, enzyme-activities, forest soils, Geology, gradient gel-electrophoresis, heavy-metals, INDUCED COMMUNITY TOLERANCE, MicroResp, microresp(tm) method, physiological profiles, populations, Soil enzymes |
| Abstract | Microbial genetic, structural and functional diversity was assessed in response to arsenic (As) pollution in a former gold mine soil. Ester-linked fatty acid methyl ester (EL-FAME), quantitative PCR (qPCR), denaturating gradient gel electrophoresis (DGGE), enzyme activities and MicroResp techniques were used.Multivariate analysis showed that As bioavailability in soil was an important driver affecting microbial diversity. Microbial biomass assessed by EL-FAMEs and qPCR generally decreased under higher bioavailable As, as well as enzyme activities and C substrate utilization. Conversely, actinomycetes and fungal biomass increased along with total As content suggesting the selection of more resistant species. |
| Short Title | Geomicrobiol. J.Geomicrobiol. J. |
| Alternate Journal | Geomicrobiol. J. |
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A Multi-biological Assay Approach to Assess Microbial Diversity in Arsenic (As) Contaminated Soils
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Stazi, S. R. Moscatelli, M. C. Papp, R. Crognale, S. Grego, S. Martin, M. Marabottini, R.
Italian Ministry of University and Research, Research Projects of National Interest PRIN [2010JBNLJ7-004]
The research was supported by the Italian Ministry of University and Research, Research Projects of National Interest PRIN 2010-2011 (2010JBNLJ7-004).
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