Coping with copper: legacy effect of copper on potential activity of soil bacteria following a century of exposure

TitleCoping with copper: legacy effect of copper on potential activity of soil bacteria following a century of exposure
Publication TypeJournal Article
Year of Publication2016
AuthorsNunes I., Jacquiod S., Brejnrod A., Holm P.E, Johansen A., Brandt K.K, Prieme A., Sorensen S.J
JournalFems Microbiology EcologyFems Microbiology EcologyFems Microbiology Ecology
Volume92
Pagination12
Date PublishedNov
Type of ArticleArticle
ISBN Number0168-6496
Accession NumberWOS:000387994800012
Keywordsbioavailable, bioindicator, copper, cu exposure, diversity, functional response group, gradient, heavy-metals, Metal toxicity, microbial communities, Microbiology, microorganisms, plfa, populations, resistance, rna, vineyard
Abstract

Copper has been intensively used in industry and agriculture since mid-18(th) century and is currently accumulating in soils. We investigated the diversity of potential active bacteria by 16S rRNA gene transcript amplicon sequencing in a temperate grassland soil subjected to century-long exposure to normal (similar to 15 mg kg(-1)), high (similar to 450 mg kg(-1)) or extremely high (similar to 4500 mg kg(-1)) copper levels. Results showed that bioavailable copper had pronounced impacts on the structure of the transcriptionally active bacterial community, overruling other environmental factors (e.g. season and pH). As copper concentration increased, bacterial richness and evenness were negatively impacted, while distinct communities with an enhanced relative abundance of Nitrospira and Acidobacteria members and a lower representation of Verrucomicrobia, Proteobacteria and Actinobacteria were selected. Our analysis showed the presence of six functional response groups (FRGs), each consisting of bacterial taxa with similar tolerance response to copper. Furthermore, the use of FRGs revealed that specific taxa like the genus Nitrospira and several Acidobacteria groups could accurately predict the copper legacy burden in our system, suggesting a potential promising role as bioindicators of copper contamination in soils.Long-term effect of a copper gradient on soil bacteria.Long-term effect of a copper gradient on soil bacteria.

Short TitleFEMS Microbiol. Ecol.FEMS Microbiol. Ecol.
Alternate JournalFEMS Microbiol. Ecol.