A Method for Determining Community Level Physiological Profiles of Organic Soil Horizons

TitleA Method for Determining Community Level Physiological Profiles of Organic Soil Horizons
Publication TypeJournal Article
Year of Publication2015
AuthorsSwallow M.JB, Quideau S.A
JournalSoil Science Society of America Journal
Volume79
Pagination536-542
Date PublishedMar-Apr
Type of ArticleArticle
ISBN Number0361-5995
Accession NumberWOS:000351640900020
Keywordsalberta, catabolic diversity, ecosystem, FOREST FLOORS, HUMUS, microbial communities, patterns, SPRUCE
Abstract

Community level physiological profiles (CLPPs) have been widely used to assess microbial community diversity in soils. Refinement of techniques to determine soil CLPP eventually led to the development of the MicroResp technique. This technique avoids many of the pitfalls of earlier methods by using a whole-soil approach coupled with the convenience of microplates. However, issues related to soil pretreatment (primarily sieving) can arise when using the standard MicroResp method to determine the CLPPs of forest floors. Here, we developed a modified multiple substrate induced respiration (multi-SIR) method that lessens the effects of pretreatment by using a larger soil volume in custom 24-well deep-well plates. Microbial community indices including catabolic evenness (E) and CLPP were determined on a range of forest soils using both the standard MicroResp and our modified multi-SIR method. The modified method reduced the variation among the triplicate substrate wells and displayed a wider range of E among the soils measured. Additionally, using multivariate nonmetric multidimensional scaling (NMDS) as well as cluster analysis, we found that the modified method was able to better detect differences in soil CLPPs. The standard MicroResp method remains a valuable technique for many soils; however, our modified multi-SIR method is more suitable for organic soils, such as forest floors, that have low bulk density.

Short TitleSoil Sci. Soc. Am. J.Soil Sci. Soc. Am. J.
Alternate JournalSoil Sci. Soc. Am. J.
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Times Cited: 0
Cited Reference Count: 35
Swallow, Mathew J. B. Quideau, Sylvie A.
Quideau, Sylvie/C-9646-2015
Quideau, Sylvie/0000-0002-2297-9024
Natural Sciences and Engineering Research Council of Canada (NSERC); Canadian Oil Sands Network for Research and Development [CRDPJ 401580-10]
The authors thank the anonymous reviewers and the associate editor for their insightful comments which greatly enhanced the quality and clarity of the manuscript. Additionally, we would like to thank the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Canadian Oil Sands Network for Research and Development for overall financial support provided through a grant (CRDPJ 401580-10) to S. Quideau.

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