| Title | Physiological, biochemical and molecular responses of the soil microbial community after afforestation of pastures with Pinus radiata |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Macdonald CA, Thomas N, Robinson L, Tate KR, Ross DJ, Dando J, Singh BK |
| Journal | Soil Biology and Biochemistry |
| Volume | 41 |
| Pagination | 1642-1651 |
| Date Published | 2009/08/01/ |
| ISBN Number | 0038-0717 |
| Keywords | Afforestation, microbial community structure, Microbial function, Soil physico-chemical properties |
| Abstract | Afforestation and deforestation are key land-use changes across the world, and are considered to be dominant factors controlling ecosystem functioning and biodiversity. However, the responses of soil microbial communities to these land-use changes are not well understood. Because changes in soil microbial abundance and community structure have consequences for nutrient cycling, C-sequestration and long-term sustainability, we investigated impacts of land-use change, age of stand and soil physico-chemical properties on fungal and bacterial communities and their metabolic activities. This study was carried out at four sites in two geographical locations that were afforested on long-established pastures with Pinus radiata D. Don (pine). Two of the sites were on volcanic soils and two on non-volcanic soils and stand age ranged from 5 to 20 y. Microbial communities were analysed by biochemical (phospho-lipid fatty acids; PLFA) and molecular (multiplex-terminal restriction fragment length polymorphism; M-TRFLP) approaches. Both site and stand age influenced microbial properties, with changes being least detectable in the 5-y-old stand. Land use was a key factor influencing soil metabolic activities as measured by physiological profiling using MicroResp. Pasture soils had higher microbial biomass (P |
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Physiological, biochemical and molecular responses of the soil microbial community after afforestation of pastures with Pinus radiata
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