The effect of chelating agents on the Zn-phytoextraction potential of hemp and soil microbial activity

TitleThe effect of chelating agents on the Zn-phytoextraction potential of hemp and soil microbial activity
Publication TypeJournal Article
Year of Publication2024
AuthorsKalousek P., Holatko J., Schreiber P., Pluhácek T., Lónová K.S, Radziemska M., Tarkowski P., Vyhnánek T., Hammerschmiedt T., Brtnicky M.
JournalChemical and Biological Technologies in Agriculture
Volume11
Pagination19
Date PublishedFeb
Type of ArticleArticle
Accession NumberWOS:001161858600001
KeywordsAgriculture, antioxidant defense system, arabidopsis-thaliana, Cannabis sativa, chlorophyll fluorescence, Citric acid, citric-acid, enhanced phytoextraction, heavy-metal contamination, induced oxidative, Nitrilotriacetic acid, Photosynthetic characteristics, photosystem-ii, phytoremediation, SEWAGE-SLUDGE, soil microorganisms, stress, zinc accumulation
Abstract

Background Hemp (Cannabis sativa) is a crop with a wide range of uses, from the production of fiber and seeds to the secondary metabolites for medicinal purposes. In addition, it is characterized by high biomass yield and the ability to accumulate heavy metals, which makes this plant convenient for phytoremediation purposes. In this study, the effect of applying exogenous biodegradable chelating agents, citric acid (CA) and nitrilotriacetic acid (NTA), to zinc-contaminated soil on zinc (Zn) uptake by two industrial hemp varieties 'Felina 32' and 'Monoica' was studied. The effect of CA and NTA on available Zn in soils was investigated using an 'in pot' experiment under controlled conditions. The effect of both tested compounds on soil microbial activity was simultaneously evaluated. Results After the application of NTA at a concentration of 5 mmol L-1, a > threefold increased accumulation of Zn in the above-ground parts was recorded in the 'Felina 32' variety. In the 'Monoica' variety, the levels of Zn in the above-ground parts were increased > twofold. NTA affected the soil microbiome negatively, causing decreased enzyme activity (in 'Monoica' planted soil) and induced respiration (in 'Monoica' and especially in 'Felina 32' planted soil). On the other hand, CA application did not lead to significantly increased Zn levels in any of the studied hemp varieties. Together with CA's negative effects on some soil enzymes, CA enhanced urease activity, dehydrogenase and several respiration types for the 'Felina 32' variety and exerted less detrimental effect on the soil microbiome. No toxic effects from increased Zn uptake and accumulation in experimental plants were detected, accounting for the unchanged physiological stress markers (levels of photosynthetic pigments and proline in leaves, chlorophyll fluorescence parameters) and selected growth traits of the above-ground organs and root system. Conclusions From the studied varieties, 'Felina 32' seems to be more suitable for Zn-phytoextraction because of its higher tolerance to increased Zn levels, higher biomass production and Zn accumulation capacity. Our results indicate the potential of using the 'Felina 32' variety in NTA-assisted Zn phytoextraction from contaminated soils.

Alternate JournalChem. Biol. Technol. Agric.
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    [biblio_abst_e] => Background Hemp (Cannabis sativa) is a crop with a wide range of uses, from the production of fiber and seeds to the secondary metabolites for medicinal purposes. In addition, it is characterized by high biomass yield and the ability to accumulate heavy metals, which makes this plant convenient for phytoremediation purposes. In this study, the effect of applying exogenous biodegradable chelating agents, citric acid (CA) and nitrilotriacetic acid (NTA), to zinc-contaminated soil on zinc (Zn) uptake by two industrial hemp varieties 'Felina 32' and 'Monoica' was studied. The effect of CA and NTA on available Zn in soils was investigated using an 'in pot' experiment under controlled conditions. The effect of both tested compounds on soil microbial activity was simultaneously evaluated. Results After the application of NTA at a concentration of 5 mmol L-1, a > threefold increased accumulation of Zn in the above-ground parts was recorded in the 'Felina 32' variety. In the 'Monoica' variety, the levels of Zn in the above-ground parts were increased > twofold. NTA affected the soil microbiome negatively, causing decreased enzyme activity (in 'Monoica' planted soil) and induced respiration (in 'Monoica' and especially in 'Felina 32' planted soil). On the other hand, CA application did not lead to significantly increased Zn levels in any of the studied hemp varieties. Together with CA's negative effects on some soil enzymes, CA enhanced urease activity, dehydrogenase and several respiration types for the 'Felina 32' variety and exerted less detrimental effect on the soil microbiome. No toxic effects from increased Zn uptake and accumulation in experimental plants were detected, accounting for the unchanged physiological stress markers (levels of photosynthetic pigments and proline in leaves, chlorophyll fluorescence parameters) and selected growth traits of the above-ground organs and root system. Conclusions From the studied varieties, 'Felina 32' seems to be more suitable for Zn-phytoextraction because of its higher tolerance to increased Zn levels, higher biomass production and Zn accumulation capacity. Our results indicate the potential of using the 'Felina 32' variety in NTA-assisted Zn phytoextraction from contaminated soils.
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Times Cited: 1
Cited Reference Count: 120
Kalousek, Petr Holatko, Jiri Schreiber, Patrik Pluhacek, Tomas Lonova, Kamila Siruckova Radziemska, Maja Tarkowski, Petr Vyhnanek, Tomas Hammerschmiedt, Tereza Brtnicky, Martin
Lónová, Kamila/AAG-7003-2021; Holatko, Jiri/AAX-5624-2020; Brtnicky, Martin/I-4939-2018; Hammerschmiedt, Tereza/W-5891-2018; Vyhnanek, Tomas/D-5247-2012
Lónová, Kamila/0000-0002-8616-4289; Holatko, Jiri/0000-0003-4156-4673; Brtnicky, Martin/0000-0001-5237-722X; Hammerschmiedt, Tereza/0000-0003-1621-2019; Kalousek, Petr/0000-0003-3476-9443; Vyhnanek, Tomas/0000-0002-9628-3716
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