| Title | An inquiline mosquito modulates microbial diversity and function in an aquatic microecosystem |
| Publication Type | Journal Article |
| Year of Publication | 2024 |
| Authors | Arellano A.A, Young E.B, Coon K.L |
| Journal | Molecular Ecology |
| Volume | 33 |
| Pagination | 17 |
| Date Published | Apr |
| Type of Article | Article |
| ISBN Number | 0962-1083 |
| Accession Number | WOS:001178261100001 |
| Keywords | Bacteria, Biochemistry & Molecular Biology, carnivorous plant, community, Environmental Sciences & Ecology, Evolutionary Biology, expression, food webs, microbiota, mutualism, nitrogen, northern pitcher plant, nutrient degradation/cycling, plant sarracenia-purpurea, species richness, storage proteins, symbiosis, top-down, top-down control |
| Abstract | Understanding microbial roles in ecosystem function requires integrating microscopic processes into food webs. The carnivorous pitcher plant, Sarracenia purpurea, offers a tractable study system where diverse food webs of macroinvertebrates and microbes facilitate digestion of captured insect prey, releasing nutrients supporting the food web and host plant. However, how interactions between these macroinvertebrate and microbial communities contribute to ecosystem functions remains unclear. We examined the role of the pitcher plant mosquito, Wyeomyia smithii, in top-down control of the composition and function of pitcher plant microbial communities. Mosquito larval abundance was enriched or depleted across a natural population of S. purpurea pitchers over a 74-day field experiment. Bacterial community composition and microbial community function were characterized by 16S rRNA amplicon sequencing and profiling of carbon substrate use, bulk metabolic rate, hydrolytic enzyme activity, and macronutrient pools. Bacterial communities changed from pitcher opening to maturation, but larvae exerted minor effects on high-level taxonomic composition. Higher larval abundance was associated with lower diversity communities with distinct functions and elevated nitrogen availability. Treatment-independent clustering also supported roles for larvae in curating pitcher microbial communities through shifts in community diversity and function. These results demonstrate top-down control of microbial functions in an aquatic microecosystem.
|
| Short Title | Mol. Ecol.Mol. Ecol. |
| Alternate Journal | Mol. Ecol. |
stdClass Object
(
[vid] => 890
[uid] => 11
[title] => An inquiline mosquito modulates microbial diversity and function in an aquatic microecosystem
[log] =>
[status] => 1
[comment] => 0
[promote] => 1
[sticky] => 0
[nid] => 708
[type] => biblio
[language] => und
[created] => 1718019118
[changed] => 1718019118
[tnid] => 0
[translate] => 0
[revision_timestamp] => 1718019118
[revision_uid] => 11
[biblio_type] => 102
[biblio_number] => 7
[biblio_other_number] =>
[biblio_sort_title] => inquiline mosquito modulates microbial diversity and function in
[biblio_secondary_title] => Molecular Ecology
[biblio_tertiary_title] =>
[biblio_edition] =>
[biblio_publisher] =>
[biblio_place_published] =>
[biblio_year] => 2024
[biblio_volume] => 33
[biblio_pages] => 17
[biblio_date] => Apr
[biblio_isbn] => 0962-1083
[biblio_lang] => English
[biblio_abst_e] => Understanding microbial roles in ecosystem function requires integrating microscopic processes into food webs. The carnivorous pitcher plant, Sarracenia purpurea, offers a tractable study system where diverse food webs of macroinvertebrates and microbes facilitate digestion of captured insect prey, releasing nutrients supporting the food web and host plant. However, how interactions between these macroinvertebrate and microbial communities contribute to ecosystem functions remains unclear. We examined the role of the pitcher plant mosquito, Wyeomyia smithii, in top-down control of the composition and function of pitcher plant microbial communities. Mosquito larval abundance was enriched or depleted across a natural population of S. purpurea pitchers over a 74-day field experiment. Bacterial community composition and microbial community function were characterized by 16S rRNA amplicon sequencing and profiling of carbon substrate use, bulk metabolic rate, hydrolytic enzyme activity, and macronutrient pools. Bacterial communities changed from pitcher opening to maturation, but larvae exerted minor effects on high-level taxonomic composition. Higher larval abundance was associated with lower diversity communities with distinct functions and elevated nitrogen availability. Treatment-independent clustering also supported roles for larvae in curating pitcher microbial communities through shifts in community diversity and function. These results demonstrate top-down control of microbial functions in an aquatic microecosystem.
[biblio_abst_f] =>
[biblio_full_text] => 0
[biblio_url] =>
[biblio_issue] =>
[biblio_type_of_work] => Article
[biblio_accession_number] => WOS:001178261100001
[biblio_call_number] =>
[biblio_notes] => ISI Document Delivery No.: MA6F9
Times Cited: 0
Cited Reference Count: 98
Arellano, Aldo A. Young, Erica B. Coon, Kerri L.
Arellano, Aldo/0000-0002-3780-1130
Howard Hughes Medical Institute; Bog State Natural Area in Wisconsin
We thank Gretchen Meyer and the University of Wisconsin-Milwaukee Field Station for access to field sampling at the Cedarburg Bog State Natural Area in Wisconsin. We also thank Jacob Grothjan for assistance with hydrolytic enzyme assays conducted at the University of Wisconsin-Milwaukee, along with T. Miller and a second, anonymous reviewer for their thoughtful and helpful feedback.
3
Wiley
Hoboken
1365-294x
[biblio_custom1] =>
[biblio_custom2] =>
[biblio_custom3] =>
[biblio_custom4] =>
[biblio_custom5] =>
[biblio_custom6] =>
[biblio_custom7] =>
[biblio_research_notes] =>
[biblio_number_of_volumes] =>
[biblio_short_title] => Mol. Ecol.Mol. Ecol.
[biblio_alternate_title] => Mol. Ecol.
[biblio_original_publication] =>
[biblio_reprint_edition] =>
[biblio_translated_title] =>
[biblio_section] =>
[biblio_citekey] => 708
[biblio_coins] =>
[biblio_doi] =>
[biblio_issn] =>
[biblio_auth_address] => [Arellano, Aldo A.] Univ Wisconsin, Microbiol Doctoral Training Program, Madison, WI USA. [Arellano, Aldo A.; Coon, Kerri L.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Young, Erica B.] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI USA. [Young, Erica B.] Univ Wisconsin, Sch Freshwater Sci, Milwaukee, WI USA. University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Milwaukee; University of Wisconsin System; University of Wisconsin Milwaukee
Coon, KL (corresponding author), Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA.
kerri.coon@wisc.edu
[biblio_remote_db_name] =>
[biblio_remote_db_provider] =>
[biblio_label] =>
[biblio_access_date] =>
[biblio_refereed] =>
[biblio_md5] => f50cfe4100b976c41fc802eb678f3386
[biblio_formats] => Array
(
[biblio_abst_e] => full_html
[biblio_abst_f] => full_html
[biblio_notes] => full_html
[biblio_research_notes] => full_html
[biblio_custom1] => full_html
[biblio_custom2] => full_html
[biblio_custom3] => full_html
[biblio_custom4] => full_html
[biblio_custom5] => full_html
[biblio_custom6] => full_html
[biblio_custom7] => full_html
[biblio_coins] => full_html
[biblio_auth_address] => full_html
)
[biblio_type_name] => Journal Article
[biblio_contributors] => Array
(
[0] => Array
(
[nid] => 708
[vid] => 890
[cid] => 2066
[auth_type] => 1
[auth_category] => 1
[rank] => 0
[merge_cid] => 0
[aka] => 0
[alt_form] => 0
[drupal_uid] =>
[name] => Arellano, A. A.
[lastname] => Arellano
[firstname] => A.
[prefix] =>
[suffix] =>
[initials] => A.
[affiliation] =>
[literal] => 0
[md5] => 19dad3101b25f99652e828cfe9f169ef
)
[1] => Array
(
[nid] => 708
[vid] => 890
[cid] => 2067
[auth_type] => 1
[auth_category] => 1
[rank] => 1
[merge_cid] => 0
[aka] => 0
[alt_form] => 0
[drupal_uid] =>
[name] => Young, E. B.
[lastname] => Young
[firstname] => E.
[prefix] =>
[suffix] =>
[initials] => B.
[affiliation] =>
[literal] => 0
[md5] => 5c107307e9498005c07b1be1734b13dd
)
[2] => Array
(
[nid] => 708
[vid] => 890
[cid] => 2068
[auth_type] => 1
[auth_category] => 1
[rank] => 2
[merge_cid] => 0
[aka] => 0
[alt_form] => 0
[drupal_uid] =>
[name] => Coon, K. L.
[lastname] => Coon
[firstname] => K.
[prefix] =>
[suffix] =>
[initials] => L.
[affiliation] =>
[literal] => 0
[md5] => 21a698759f3ce810ae0b2284992b2217
)
)
[biblio_keywords] => Array
(
[117] => Bacteria
[1089] => Biochemistry & Molecular Biology
[2403] => carnivorous plant
[761] => community
[450] => Environmental Sciences & Ecology
[698] => Evolutionary Biology
[2409] => expression
[1122] => food webs
[2404] => microbiota
[2410] => mutualism
[68] => nitrogen
[2407] => northern pitcher plant
[2405] => nutrient degradation/cycling
[756] => plant sarracenia-purpurea
[1222] => species richness
[2408] => storage proteins
[1691] => symbiosis
[759] => top-down
[2406] => top-down control
)
[body] => Array
(
)
[rdf_mapping] => Array
(
[rdftype] => Array
(
[0] => sioc:Item
[1] => foaf:Document
)
[title] => Array
(
[predicates] => Array
(
[0] => dc:title
)
)
[created] => Array
(
[predicates] => Array
(
[0] => dc:date
[1] => dc:created
)
[datatype] => xsd:dateTime
[callback] => date_iso8601
)
[changed] => Array
(
[predicates] => Array
(
[0] => dc:modified
)
[datatype] => xsd:dateTime
[callback] => date_iso8601
)
[body] => Array
(
[predicates] => Array
(
[0] => content:encoded
)
)
[uid] => Array
(
[predicates] => Array
(
[0] => sioc:has_creator
)
[type] => rel
)
[name] => Array
(
[predicates] => Array
(
[0] => foaf:name
)
)
[comment_count] => Array
(
[predicates] => Array
(
[0] => sioc:num_replies
)
[datatype] => xsd:integer
)
[last_activity] => Array
(
[predicates] => Array
(
[0] => sioc:last_activity_date
)
[datatype] => xsd:dateTime
[callback] => date_iso8601
)
)
[name] => clare.cameron
[picture] => 0
[data] => a:13:{s:16:"ckeditor_default";s:1:"t";s:20:"ckeditor_show_toggle";s:1:"t";s:14:"ckeditor_width";s:4:"100%";s:13:"ckeditor_lang";s:2:"en";s:18:"ckeditor_auto_lang";s:1:"t";s:19:"biblio_show_profile";i:0;s:19:"biblio_my_pubs_menu";i:0;s:21:"biblio_contributor_id";s:1:"0";s:22:"biblio_id_change_count";s:1:"0";s:17:"biblio_user_style";s:6:"system";s:18:"biblio_baseopenurl";s:0:"";s:18:"biblio_openurl_sid";s:0:"";s:19:"biblio_crossref_pid";s:0:"";}
[entity_view_prepared] => 1
)