| Title | Combining microdilution with MicroResp™: Microbial substrate utilization, antimicrobial susceptibility and respiration |
| Publication Type | Journal Article |
| Year of Publication | 2012 |
| Authors | Drage S., Engelmeier D., Bachmann G., Sessitsch A., Mitter B., Hadacek F. |
| Journal | Journal of Microbiological Methods |
| Volume | 88 |
| Pagination | 399-412 |
| Date Published | Mar |
| Type of Article | Article |
| ISBN Number | 0167-7012 |
| Accession Number | WOS:000302666000009 |
| Keywords | antimicrobial broth-based assay, Bacteria, Biochemistry & Molecular Biology, communities, dose responses, escherichia-coli, growth, growth efficiency, hormesis, Microbiology, non-linear dose-response effects, physiological profiles, rates, respiration, stimulation, stress |
| Abstract | Pharmacological studies focus on susceptibility of pathogenic microbes against specific drugs or combinations of them, ecological studies on substrate utilization efficiency of variable microbial communities. The Micro-Resp (TM) system was especially developed to study soil microbial communities. It was slightly modified to facilitate exploring of microbial growth efficiency in a concentration-dependent fashion (microdilutions of carbohydrate mixtures or specific toxic chemicals). After turbidimetric growth assessment, calorimetric indicator plates (cresol red agar) were mounted to the assay plates. The substrate utilisation design is illustrated by glucose and a plant carbohydrate mixture, the antimicrobial susceptibility design by the naphthoquinone juglone. Dose-response effects are explored by curve fitting of nonlinear models that especially have been developed to detect hormetic effects that are characterized by stimulation at lower followed by inhibition at higher dosages (U- and inverse U-shaped effects). Multivariate analyses are presented utilizing metavariables that were obtained in the curve fitting process of the measured parameters growth and respiration and the factor growth efficiency. (C) 2012 Elsevier B V. All rights reserved.
|
| Short Title | J Microbiol Meth |
| Alternate Journal | J. Microbiol. Methods |
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Times Cited: 10
Cited Reference Count: 44
Drage, Sigrid Engelmeier, Doris Bachmann, Gert Sessitsch, Angela Mitter, Birgit Hadacek, Franz
Bachmann, Gert/C-1425-2014; Hadacek, Franz/AFR-2735-2022; Sessitsch, Angela/AAW-3191-2020
Sessitsch, Angela/0000-0003-0137-930X
Austrian Science Fund (FWF) [P19851-P17]; Austrian Science Fund (FWF) [P19851] Funding Source: Austrian Science Fund (FWF)
This research was supported by grant P19851-P17 of the Austrian Science Fund (FWF) to BM and FH.
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