Distance-dependent varieties of microbial community structure and metabolic functions in the rhizosphere of Sedum alfredii Hance during phytoextraction of a cadmium-contaminated soil

TitleDistance-dependent varieties of microbial community structure and metabolic functions in the rhizosphere of Sedum alfredii Hance during phytoextraction of a cadmium-contaminated soil
Publication TypeJournal Article
Year of Publication2017
AuthorsYang WH, Zhang TX, Lin S, Ni WZ
JournalEnvironmental Science and Pollution Research
Volume24
Pagination14234-14248
Date PublishedJun
Type of ArticleArticle
ISBN Number0944-1344
Accession NumberWOS:000402748300030
Keywordscadmium, chemical-changes, different amendments, Environmental Sciences & Ecology, grassland ecosystems, heavy-metals, metal-polluted soils, method, microbial community, microresp (tm), MICRORESP(TM), mycorrhizal fungi, physiological, Phytoextraction, plfa, PROFILES, Rhizospheric, substrate utilization, thlaspi-caerulescens
Abstract

The recovery of microbial community and activities is crucial to the remediation of contaminated soils. Distancedependent variations ofmicrobial community composition and metabolic characteristics in the rhizospheric soil of hyperaccumulator during phytoextraction are poorly understood. A 12-month phytoextraction experiment with Sedum alfredii in a Cd-contaminated soil was conducted. A prestratified rhizobox was used for separating sub-layer rhizospheric (0-2, 2-4, 4-6, 6-8, 8-10 mm from the root mat)/bulk soils. Soil microbial structure and function were analyzed by phospholipid fatty acid (PLFA) and MicroResp T methods. The concentrations of total and specified PLFA biomarkers and the utilization rates for the 14 substrates (organic carbon) in the 0-2-mm sub-layer rhizospheric soil were significantly increased, as well as decreased with the increase in the distance from the root mat. Microbial structure measured by the ratios of different groups of PLFAs such as fungal/bacterial, monounsaturated/saturated, ratios of Gram-positive to Gram-negative (GP/GN) bacterial, and cyclopropyl/monoenoic precursors and 19:0 cyclo/18:1 omega 7c were significantly changed in the 0-2-mm soil. The PLFA contents and substrate utilization rates were negatively correlated with pH and total, acid-soluble, and reducible fractions of Cd, while positively correlated with labile carbon. The dynamics of microbial community were likely due to root exudates and Cd uptake by S. alfredii. This study revealed the stimulations and gradient changes of rhizosphere microbial community through phytoextraction, as reduced Cd concentration, pH, and increased labile carbons are due to the microbial community responses.

Alternate JournalEnviron. Sci. Pollut. Res.