MicroResp™ Procedure in Brief

 Colorimetic method

Detection Plate Preparation Prepare Detection plates and store in sealed container/dessicator with self-indicating soda lime.
Dispense Carbon Sources Prepare carbon sources and dispense into Deepwell plate.

If you have lots of soil samples to measure it is easier to fill the Deepwell plates prior to the addition of the carbon sources.

Half Filled Filling Device Place Filling Device over Deepwell plate and fill with soil. Multiple soil samples can be used by using masking tape to cover the wells that are not to be filled.
Filled Filling Device Remove sliding perspex sheet to allow soil to fall into Deepwell plate. Assist the soil by holding the Deepwell plate with the Filling Device and tapping gently but firmly .
MicroResp System Assembled Read Detection plate at 570nm and assemble onto the Deepwell plate with the MicroResp™ seal.
MicroResp System in Clamp Secure in metal clamp and incubate at 25°C for 6 hours before re-reading the Detection plate at 570nm.

 Radioactive Carbon Method

14C-Detection Plate close-up Prepare 14C sources and 14C-Detection plates - strips of filter paper rolled and inserted into each well of a Isoplate-96 microplate.
Dispense 14-C sources Dispense 14C sources into Deepwell plate. Add NaOH to 14C-Detection plate.
Filling Device filled Place Filling device over Deepwell plate and fill with soil. Multiple soil samples can be used by using masking tape to cover the wells that are not to be filled. Remove sliding perspex sheet to allow soil to fall into Deepwell plate. Assist the soil by holding the Deepwell plate with the Filling Device and tapping gently but firmly .
14-C MicroResp Assemble the 14C-Detection plate onto the Deepwell plate with the MicroResp™ seal.
14-C MicroResp in Clamp Secure in metal clamp and incubate at 25°C for 6 hours.
  Remove 14C-Detection plate and add scintillation fluid. Seal the 14C-Detection plate with a self-adhesive microplate seal. Read the 14C-Detection plate as per instructions for the scintillation counter plate-reader.